Earl W. Davie

University of Washington


Election Year: 1980
Primary Section: 21, Biochemistry
Membership Type: Member

Research Interests

The research of my group deals with the plasma proteins involved in blood coagulation and fibrinolysis. In this research, the structure and function of a number of purified plasma proteins are being studied and their role in the formation of fibrin examined. This work has involved the isolation and characterization of these glycoproteins and a detailed study of their mechanism of activation. The genes and cDNAs coding for these proteins have also been isolated and characterized. Nearly all of these proteins are synthesized in the liver and then secreted into the blood. Accordingly, they are synthesized with a leader sequence and subsequently undergo considerable processing, including glycosylation, proteolytic modification, carboxylation, and hydroxylation. Furthermore, proteolytic enzymes of tissue origin involved in the activation of the coagulation cascade and processing enzymes involved in the maturation of these plasma proteins are studied. The isolation and characterization of the genes coding for these proteins has also made it possible to study a number of abnormal human genes in order to identify abnormalities at the DNA level. Furthermore, a number of these proteins have been expressed in mammalian cell culture in order to study their assembly, structure, and function. These studies also have included site-specific mutagenesis to identify important functional portions of several coagulation proteins, including fibrinogen, factor X, and factor XI.

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