James E. Dahlberg

My research is in cell biology and the regulation of gene expression. Currently, I am studying the mechanisms by which RNA and protein molecules are transported to -- and localized within -- the nucleus or cytoplasm of eukaryotic cells. Our long-term goal is to gain a detailed molecular understanding of the mechanism and control of macromolecular transport into and out of the nucleus. Most of our work on RNA metabolism has been carried out in the model systems synthesis, transport, and maturation of tRNAs and snRNAs in vertebrate tissue culture cells, and in the oocytes of frogs (Xenopus laevis). We are also asking how nucleo-cytoplasmic transport is controlled. To ask these questions, we use specific inhibitors of various transport functions such as antibodies to nucleoporins, synthetic peptides, and viral gene products.